Technology

DNA SEQUENCING

High-throughput next generation sequencing (NGS) has revolutionized traditional genomic methods. NGS allows researchers to perform complex and large-scale genomic research accessibly and at an affordable cost, including those exploring the human genome. It has also continually improved capacity, sensitivity, and accuracy, while lowering cost and turnaround time. We help accelerate genomic and translational research by offering high quality DNA sequencing services to institutions and biopharma. Our expertise ensures that our clients all over the world receive informative and accurate datasets with a competitive turnaround time.  

See Our Workflow and Platforms

WHAT WE OFFER

Whole Genome Sequencing (WGS)

WGS determines an organism’s complete DNA sequence in a single test.  Human WGS is not only a research tool, but is also being introduced into clinics to advance personalized medicine. It captures both large scale genomic alterations and small variants at the single nucleotide level. WGS sheds light on pathogenic and susceptibility gene detection in cancer and other diseases, while facilitating genome-wide association studies and evolutionary analysis.   

Whole Genome Bisulfite Sequencing (WGBS)

WGBS detects the DNA methylation status of single cytosines at the genome-wide level, by treating genomic DNA with sodium bisulfite before sequencing. Unmethylated cytosines appear as thymines after sequencing, while methylated cytosines are not converted. DNA methylation is an epigenomic modification that plays an important role in gene regulation, development and tumorigenesis. WGBS has been used as an effective tool for the early diagnosis of diseases, and in developmental biology research, forensic science and many other applications 

Human Whole Exome Sequencing (WES)

WES targets the exome – the proteincoding region of the genome. The human exome makes up ~2% of the whole genome, while containing approximately 85% of disease variants, making the test more efficient at detecting rare and low frequency variants compared to targeted hotspot gene panels. WES is also more cost-effective when WGS is not practical or necessary. We offer different options for exome enrichment, from coding exons only to expanded panels with untranslated regions (UTRs).  

OUR WORKFLOW

A one-stop solution to your research

  • DNA sample quality control 
  • Library construction and quality control 
  • High-throughput sequencing 
  • Standard or customized bioinformatic analysis 
  • Secure cloud-based data delivery  
TURNAROUND TIME
  • 2-4 weeks* from receipt of samples in our sequencing facility 
*Longer turnaround time may be needed if a large number of samples are submitted. 
SAMPLE TYPES

Extracted DNA

SEQUENCING PERFORMANCE SPECIFICATIONS
  • Platforms: HiSeq X-TEN or NovaSeq 6000 
  • Read length: Pair-end 2 x 150bp 
  • Quality score: >80% of bases higher than Q30 

Sample Requisition

Sample Type 

Concentration* 

Total amount 

Other requirements 

Preferred Buffer 

Genomic DNA  ≥ 10 ng/ul  ≥250 ng  A260/280: 1.7-2.0 

A260/230: 1.8-2.0 

EB or TE buffer 
Cell free DNA  ≥ 0.5 ng/ul  ≥25 ng  Fragment size between 100~500bp with the peak at 130-170bp (no genomic DNA contamination)  EB or TE buffer 
*Sample quantity should be determined by fluorometric method (e.g. Qubit, PicoGreen). 

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