DNA SEQUENCING
High-throughput next generation sequencing (NGS) has revolutionized traditional genomic methods. NGS allows researchers to perform complex and large-scale genomic research accessibly and at an affordable cost, including those exploring the human genome. It has also continually improved capacity, sensitivity, and accuracy, while lowering cost and turnaround time. We help accelerate genomic and translational research by offering high quality DNA sequencing services to institutions and biopharma. Our expertise ensures that our clients all over the world receive informative and accurate datasets with a competitive turnaround time.
DNA Samples Ready Made Library
WHAT WE OFFER
Whole Genome Sequencing (WGS)
WGS determines an organism’s complete DNA sequence in a single test. Human WGS is not only a research tool, but is also being introduced into clinics to advance personalized medicine. It captures both large scale genomic alterations and small variants at the single nucleotide level. WGS sheds light on pathogenic and susceptibility gene detection in cancer and other diseases, while facilitating genome-wide association studies and evolutionary analysis.
Human Whole Exome Sequencing (WES)
WES targets the exome – the protein–coding region of the genome. The human exome makes up ~2% of the whole genome, while containing approximately 85% of disease variants, making the test more efficient at detecting rare and low frequency variants compared to targeted hotspot gene panels. WES is also more cost-effective when WGS is not practical or necessary. We offer different options for exome enrichment, from coding exons only to expanded panels with untranslated regions (UTRs).
OUR WORKFLOW
A one-stop solution to your research
- DNA sample quality control
- Library construction and quality control
- High-throughput sequencing
- Standard or customized bioinformatic analysis
- Secure cloud-based data delivery
TURNAROUND TIME
- 2-4 weeks* from receipt of samples in our sequencing facility
*Longer turnaround time may be needed if a large number of samples is submitted.
SAMPLE TYPE
Extracted DNA
SEQUENCING PERFORMANCE SPECIFICATIONS
- Read length: Pair-end 2 x 150bp
- Quality score: >80% of bases higher than Q30
Sample Requisition - DNA Samples
| Service | Sample Type | Concentration* | Total amount | Other requirements | Preferred Buffer | Preferred Storage Method |
| WGS | Genomic DNA | ≥ 10 ng/ul | 20-1000 ng | Volume ≥ 10ul
A260/280: 1.7-2.0 A260/230: 1.7-2.0 |
EB or TE buffer | 1.5ml DNA lobind micro-centrifuge tube |
| Cell free DNA | ≥ 1 ng/ul | 5-200 ng | Volume ≥ 10ul
Fragment size between 100~350bp (no genomic DNA contamination) |
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| WES | Genomic DNA | ≥ 20 ng/ul | 200-1000 ng | Volume ≥ 10ul
A260/280: 1.7-2.0 A260/230: 1.7-2.0 |
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| Cell free DNA | ≥ 1 ng/ul | 25-200 ng | Volume ≥ 10ul
Fragment size between 100~350bp (no genomic DNA contamination) |
*Sample quantity should be determined by fluorometric method (e.g. Qubit, PicoGreen).
Ready-made Library Sequencing
Sequencing your libraries at competitive prices and fast turnaround times
SAMPLE TYPE
DNA/cDNA library
TURNAROUND TIME
*Longer turnaround time may be needed if a large number of samples are submitted
2-4 weeks* from receipt of samples in our sequencing facility
SEQUENCING PERFORMANCE SPECIFICATIONS
- Read length: Pair-end 2x150bp
- Quality score: >80% of bases higher than Q30
Sample Requisition - Ready-Made Library
Platform |
Concentration* |
Volume** |
Fragment size |
Preferred Buffer |
Preferred Storage Method |
Illumina |
≥ 5nM |
≥ 30ul |
90% of library fragments
|
EB or TE buffer |
1.5ml DNA lobind micro-centrifuge tube |
Complete Genomics |
≥ 6.5nM |
≥ 20ul |
*Sample quantity should be determined by fluorometric method (e.g. Qubit, PicoGreen).
**The total volume of the library pool for one full lane on the flow cell
LET’S ACCELERATE PRECISION CANCER CARE, TOGETHER.
Learn more about our products and services or contact us today to discuss how we can partner together.
