Technology

OTHER SERVICES

We offer the following additional services to facilitate and support your research in different disciplines:

 

Ready-made Library Sequencing

 

TCR Repertoire Sequencing

Ready-made Library Sequencing

Sequencing your libraries at competitive prices and fast turnaround times

SAMPLE TYPES

DNA/cDNA library for Illumina Platform

SEQUENCING PERFORMANCE SPECIFICATIONS
  • Platforms: NovaSeq 6000
  • Read length: Pair-end 2x150bp
  • Quality score: >80% of bases higher than Q30
TURNAROUND TIME

2-4 weeks* from receipt of samples in our sequencing facility

*Longer turnaround time may be needed if a large number of samples are submitted
SAMPLE REQUISITION
Platform Concentration* Volume** Fragment size Preferred Buffer
Hiseq X-Ten ≥ 5nM or ≥2ng/ul ≥ 10ul 90% of library fragments

< 600bp

EB or TE buffer
NovaSeq 6000 ≥ 30ul
*Sample quantity should be determined by fluorometric method (e.g. Qubit, PicoGreen). 
**The total volume of the library pool for one full lane on the flow cell
What is Precision Oncology

TCR Repertoire Sequencing 

A snapshot of the TCR Repertoire with accurate and quantitative sequencing  

Diversity in the variable region of T cell receptors (TCRs) enables T cells to recognize different molecules. Its ability to detect foreign molecules comes from the recombination of V(D)J gene segments by incorporating random nucleotide insertions and deletions, and therefore plays a key role in the sophisticated adaptive immunity system. 

Next-generation sequencing of the TCR repertoire is a powerful tool that creates a snapshot of all types of TCR recombination in a specific microenvironment. They are useful for predicting disease outcome, monitoring response to therapies as well as discovering new prognostic and diagnostic biomarkers. 

Our assay utilizes a single reaction of bias-controlled multiplex PCR approach, followed by high-throughput sequencing to provide accurate and quantitative sequences of the human TCR repertoire. We also offer comprehensive bioinformatics analyses of TCR diversity, clonality, V/J/VJ usage and CDR3 functionality. 

SAMPLE TYPES

Extracted DNA

Peripheral blood

Icon Tumour Tissue

Tumor tissue (FFPE block/slides, or frozen tissue)

LET’S ACCELERATE PRECISION CANCER CARE, TOGETHER.

Learn more about our products and services or contact us today to discuss how we can partner together.

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