OTHER SERVICES
We offer the following additional services to facilitate and support your research in different disciplines:
Ready-made Library Sequencing
TCR Repertoire Sequencing
Ready-made Library Sequencing
Sequencing your libraries at competitive prices and fast turnaround times
SAMPLE TYPES
DNA/cDNA library for Illumina Platform
SEQUENCING PERFORMANCE SPECIFICATIONS
- Platforms: NovaSeq 6000
- Read length: Pair-end 2x150bp
- Quality score: >80% of bases higher than Q30
TURNAROUND TIME
2-4 weeks* from receipt of samples in our sequencing facility
*Longer turnaround time may be needed if a large number of samples are submitted
SAMPLE REQUISITION
| Platform | Concentration* | Volume** | Fragment size | Preferred Buffer |
| Hiseq X-Ten | ≥ 5nM or ≥2ng/ul | ≥ 10ul | 90% of library fragments
< 600bp |
EB or TE buffer |
| NovaSeq 6000 | ≥ 30ul |
*Sample quantity should be determined by fluorometric method (e.g. Qubit, PicoGreen).
**The total volume of the library pool for one full lane on the flow cell
TCR Repertoire Sequencing
A snapshot of the TCR Repertoire with accurate and quantitative sequencing
Diversity in the variable region of T cell receptors (TCRs) enables T cells to recognize different molecules. Its ability to detect foreign molecules comes from the recombination of V(D)J gene segments by incorporating random nucleotide insertions and deletions, and therefore plays a key role in the sophisticated adaptive immunity system.
Next-generation sequencing of the TCR repertoire is a powerful tool that creates a snapshot of all types of TCR recombination in a specific microenvironment. They are useful for predicting disease outcome, monitoring response to therapies as well as discovering new prognostic and diagnostic biomarkers.
Our assay utilizes a single reaction of bias-controlled multiplex PCR approach, followed by high-throughput sequencing to provide accurate and quantitative sequences of the human TCR repertoire. We also offer comprehensive bioinformatics analyses of TCR diversity, clonality, V/J/VJ usage and CDR3 functionality.
SAMPLE TYPES
Extracted DNA
Peripheral blood
Tumor tissue (FFPE block/slides, or frozen tissue)
LET’S ACCELERATE PRECISION CANCER CARE, TOGETHER.
Learn more about our products and services or contact us today to discuss how we can partner together.