OTHER SERVICES
We offer the following additional services to facilitate and support your research in different disciplines:
Ready-made Library Sequencing
TCR Repertoire Sequencing
Single-cell Transcriptome Sequencing
Ready-made Library Sequencing
Sequencing your libraries at competitive prices and fast turnaround times
SAMPLE TYPES
DNA/cDNA library for Illumina Platform
SEQUENCING PERFORMANCE SPECIFICATIONS
- Platforms: HiSeq X-TEN or NovaSeq 6000
- Read length: Pair-end 2x150bp
- Quality score: >80% of bases higher than Q30
TURNAROUND TIME
2-4 weeks* from receipt of samples in our sequencing facility
*Longer turnaround time may be needed if a large number of samples are submitted
SAMPLE REQUISITION
| Platform | Concentration* | Volume** | Fragment size | Preferred Buffer |
| Hiseq X-Ten | ≥ 5nM or ≥2ng/ul | ≥ 10ul | 90% of library fragments
< 600bp |
EB or TE buffer |
| NovaSeq 6000 | ≥ 30ul |
*Sample quantity should be determined by fluorometric method (e.g. Qubit, PicoGreen).
**The total volume of the library pool for one full lane on the flow cell
TCR Repertoire Sequencing
A snapshot of the TCR Repertoire with accurate and quantitative sequencing
Diversity in the variable region of T cell receptors (TCRs) enables T cells to recognize different molecules. Its ability to detect foreign molecules comes from the recombination of V(D)J gene segments by incorporating random nucleotide insertions and deletions, and therefore plays a key role in the sophisticated adaptive immunity system.
Next-generation sequencing of the TCR repertoire is a powerful tool that creates a snapshot of all types of TCR recombination in a specific microenvironment. They are useful for predicting disease outcome, monitoring response to therapies as well as discovering new prognostic and diagnostic biomarkers.
Our assay utilizes a single reaction of bias-controlled multiplex PCR approach, followed by high-throughput sequencing to provide accurate and quantitative sequences of the human TCR repertoire. We also offer comprehensive bioinformatics analyses of TCR diversity, clonality, V/J/VJ usage and CDR3 functionality.
SAMPLE TYPES
Extracted DNA
Peripheral blood
Tumor tissue (FFPE block/slides, or frozen tissue)
Single-cell transcriptome sequencing
A high-resolution view of cellular diversity at the single cell level
Compared to traditional RNA sequencing from bulk cells, single-cell transcriptome sequencing utilizes advanced barcoding and NGS technology to analyze gene expression information from individual cells and provides a high-resolution view an individual cell’s function in the context of its microenvironment. It has been increasingly used in a variety of research directions:
- Reveals cellular level heterogeneity
- Identifies and characterizes rare cell populations
- Studies gene expression and co-expression patterns in individual cells
- Dissects developmental cell lineages and molecular changes during development and differentiation processes
- Discovers novel biomarkers for specific cell populations
PLATFORMS
- 10x Genomics Chromium System for cell partition and library preparation
- Illumina NGS platform for sequencing
SAMPLE TYPES
Isolated cells from cell lines or fresh tissue
LET’S ACCELERATE PRECISION CANCER CARE, TOGETHER.
Learn more about our products and services or contact us today to discuss how we can partner together.